Interaction of catalase with cationic hydrogels: Influence of pH, kineticsof process and isotherms of adsorption

The adsorption interaction of catalase Penicillium vitale with cationic pol ymer hydrogels prepared from the vinyl ether of monoethanolamine was studie d at various pHs of phosphate buffer solution, interaction times and enzyme concentrations. The hydrogels behaved as anion exchange swellable gel adso rbents. The formation of stable polymer-protein complexes with high enzymat ic activity has been shown. The dependence of the interaction degree of pro tein macromolecules an swelling coefficients of gel has been found. The iso therms of protein adsorption indicate the thermodynamical irreversibility o f the process. Copyright (C) 1999 John Wiley & Sons, Ltd.