Replication fork assembly at recombination intermediates is required for bacterial growth

Citation
Ji. Liu et al., Replication fork assembly at recombination intermediates is required for bacterial growth, P NAS US, 96(7), 1999, pp. 3552-3555
Citations number
24
Categorie Soggetti
Multidisciplinary
Journal title
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN journal
00278424 → ACNP
Volume
96
Issue
7
Year of publication
1999
Pages
3552 - 3555
Database
ISI
SICI code
0027-8424(19990330)96:7<3552:RFAARI>2.0.ZU;2-H
Abstract
PriA, a 3' --> 5' DNA helicase, directs assembly of a primosome on some bac teriophage and plasmid DNAs, Primosomes are multienzyme replication machine s that contribute both the DNA-unwinding and Oliazaki fragment-priming func tions at the replication fork. The role of PriA in chromosomal replication is unclear. The phenotypes of priA null mutations suggest that the protein participates in replication restart at recombination intermediates. We show here that PriA promotes replication fork assembly at a D loop, an intermed iate formed during initiation of homologous recombination. We also show tha t DnaC810, encoded by a naturally arising intergenic suppressor allele of t he priA2::kan mutation, bypasses the need for PriA during replication fork assembly at D loops in vitro. These findings underscore the essentiality of replication fork restart at recombination intermediates under normal growt h conditions in bacteria.