Interaction of mammalian cell culture broth with adsorbents in expanded bed adsorption of monoclonal antibodies

The interaction of a mammalian cell culture broth with two commercially ava ilable adsorbents for the use in expanded bed adsorption (EBA) has been stu died. A cation exchange resin (Streamline SP) and an affinity adsorbent (St reamline rProtein A) were compared with regard to adsorption of hybridoma c ells during sample application as well as potential cell damage. The result s showed that hybridoma cells interact significantly with an expanded bed o f cation exchange adsorbents but not with the Protein A adsorbent. After ap plication of 17-20 sedimented bed volumes a saturation of the Streamline SP resin with cells was noted. With both adsorbents no measurable cell damage was found acid IgG, was recovered in approximately 95% yield. The capacity for IgG, adsorption at 3% breakthrough was 2.7 mg IgG(1)/ml Streamline rPr otein A at a constant fluid velocity of 380 cm/h and 1.0 mg IgG(1)/ml Strea mline SP at 215-240 cm/h fluid velocity. (C) 1999 Elsevier Science Ltd. All rights reserved.