LOCALIZED PROTON MAGNETIC-RESONANCE SPECTROSCOPY OF THE BRAIN DIFFERENTIATES THE INBORN METABOLIC ENCEPHALOPATHIES IN CHILDREN

Localized brain proton magnetic resonance spectroscopy (MRS) has been performed using a STEAM (stimulated echo-acquisition mode) method with a short-echo time (20ms) in 10 children suffering from different lyso somal diseases, 6 boys with X-linked adrenoleukodystrophy (X-ALD) and 5 healthy children. Metabolic data from localized spectra were process ed by principal component analysis (PCA) of 7 metabolic variables reco rded on the MR spectra. PCA allows to delineate different clusters cor responding to the 2 pathological groups which are separated from each other and from the control group. The position of each spectrum on the patient map correlates with the clinical data and to the evolution of the patients subjected to a follow-up. These results also confirm the metabolic features characterizing the pathologies of the lysosome (in crease in inositol) and the peroxisome (increase in choline and free l ipids). PCA constitutes an alternative to the classical statistical me thods to analyze and compare metabolic modifications in small populati ons of patients and allows to identify the most critical parameters de fining the organization of the pathological populations. This analysis clearly increases the discrimination among pathologies based an the m etabolic profiles obtained by MRS.