J. Blasiak et al., DNA damage and repair in human lymphocytes and gastric mucosa cells exposed to chromium and curcumin, TER CAR MUT, 19(1), 1999, pp. 19-31
Human population can be considered as a subject of combined exposure to che
micals. Hexavalent chromium is a well-known mutagen and carcinogen. Curcumi
n, a popular spice and pigment, is reported to have antineoplastic properti
es. The single cell gel electrophoresis (Comet assay) is a sensitive techni
que that allows detecting double- and single-strand DNA breaks caused by a
broad spectrum of mutagens. In the present work the ability of curcumin to
reduce DNA damage induced by chromium in human lymphocytes and gastric muco
sa (GM) cells was investigated by using the comet assay. Chromium at 500 mu
M evoked DNA damage measured as significant (P < 0.001), about a two-fold
increase in comet tail moment of both lymphocytes and GM cells. Curcumin at
10, 25, and 50 mu M also damaged DNA of both types of cells in a dose-depe
ndent manner: the increase in the tail moment reached about twenty times of
the control value (P < 0.001). The combined action of chromium at 500 mu M
and curcumin at 50 mu M resulted in the significant (P < 0.001) increase i
n the comet tail moment of both types of cells. In each case, treated cells
were able to recover within 60 min. Our study clearly demonstrates that cu
rcumin does not inhibit DNA damaging action of hexavalent chromium in human
lymphocytes and GM cells. Moreover, curcumin itself can damage DNA of thes
e cells and the total effect of chromium and curcumin is additive. Further
studies are needed to establish the role of interaction of curcumin with DN
A in carcinogenesis. (C) 1999 Wiley-Liss, Inc.