Transforming growth factor (TGF)-beta mimics and anti-TGF-beta antibody abrogates the in vivo effects of cyclosporine - Demonstration of a direct role of TGF-beta in immunosuppression and nephrotoxicity of cyclosporine
Ak. Khanna et al., Transforming growth factor (TGF)-beta mimics and anti-TGF-beta antibody abrogates the in vivo effects of cyclosporine - Demonstration of a direct role of TGF-beta in immunosuppression and nephrotoxicity of cyclosporine, TRANSPLANT, 67(6), 1999, pp. 882-889
Background. Cyclosporine (CsA) has been shown to induce the expression of t
ransforming growth factor (TGF)-beta both in vitro and in vivo. It is hypot
hesized that the efficacy as well as the side effects of CsA are mediated b
y TGF-P. This study was planned to investigate whether anti-TGF-p mitigated
and TGF-P reproduced the in vivo effects of CsA to directly prove this hyp
othesis,
Methods. B6AF(1), (H2(b/k.d)) mice were divided into groups and received th
e following: CsA, vehicle (olive oil), CsA + anti-TGF-pl antibody, TGF-beta
1, or vehicle phosphate-buffered saline/bovine serum albumin. All studies
were carried out at 10 and 28 days after the last day of CsA administration
with the exception of the exogenous TGF-P experiments, which were performe
d 5 days after exogenous TGF-P administration. The efficacy was studied by
the anti-CD3-induced ex vivo proliferation of splenocytes measured by [H-3]
thymidine uptake; TGF-P protein levels were quantified by ELISA, TG;F-P, co
llagen, and fibronectin gene expression was studied using reverse transcrip
tasepolymerase chain reaction, and histopathological analysis was made on p
eriodic acid-Schiff- and trichrome C-stained thin kidney sections.
Results. CsA treatment resulted in decreased ex vivo proliferation of splen
ocytes, an increase in TGF-P protein in the sera, and renal histopathologic
al changes including tubular swelling, vacuolization, thrombotic microangio
pathy, and increased expression of TGF-P, collagen and fibronectin genes. A
ll of these findings were blocked by anti-TGF-p antibody.
Conclusion. The study demonstrates the in vivo modulation of the effects of
CsA by manipulating TGF-P levels and suggests that TGF-P at least in part
mediates CsA's beneficial and detrimental effects.