Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland

Authors
Allan, GM Mc Neilly, F Meehan, BM Kennedy, S Mackie, DP Ellis, JA Clark, EG Espuna, E Saubi, N Riera, P Botner, A Charreyre, CE
Citation
Gm. Allan et al., Isolation and characterisation of circoviruses from pigs with wasting syndromes in Spain, Denmark and Northern Ireland, VET MICROB, 66(2), 1999, pp. 115-123
Citations number
29
Categorie Soggetti
Veterinary Medicine/Animal Health",Microbiology
Journal title
VETERINARY MICROBIOLOGY
ISSN journal
03781135 → ACNP
Volume
66
Issue
2
Year of publication
1999
Pages
115 - 123
Database
ISI
SICI code
0378-1135(19990401)66:2<115:IACOCF>2.0.ZU;2-0
Abstract
A porcine circovirus (PCV) was isolated from tissues of pigs with wasting s yndromes from Spain, Denmark and N. Ireland. The antigenic profiles of thes e viruses were determined by indirect immunofluorescence assays using polyc lonal antisera and monoclonal antibodies (mAbs) prepared against previously isolated PCVs. A rapid and convenient PCR-based test was developed and use d for the genotyping of these PCV isolates. These PCV isolates were found t o be antigenically and genomically similar to previously reported isolates of PCV from pigs with wasting disease (PCV2), but distinct from the isolate of PCV from continuous PK/15 cell cultures (PCV1). (C) 1999 Elsevier Scien ce B.V. All rights reserved.