Natural phenomena underlying the operation of the PETRO (pond enhanced trea
tment and operation) system and its trickling filter (TF) in particular hav
e been elucidated. Data obtained strongly indicate that the microalgae apar
t from bacteria, fungi, protozoa and rotifers play an important role within
the TF biofilm consortium. Their stress-induced biofilm slime production u
nder heterotrophic conditions in the TF, appears to greatly facilitate floc
culation of solids which results in high performance of the TF, the clarifi
er and the system as a whole. (C) 1999 Elsevier Science Ltd. All rights res
erved.