Characterization of the histidine mutants of Kluyveromyces lactis

Citation
Bjm. Zonneveld et al., Characterization of the histidine mutants of Kluyveromyces lactis, YEAST, 15(5), 1999, pp. 377-384
Citations number
30
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
YEAST
ISSN journal
0749503X → ACNP
Volume
15
Issue
5
Year of publication
1999
Pages
377 - 384
Database
ISI
SICI code
0749-503X(19990330)15:5<377:COTHMO>2.0.ZU;2-M
Abstract
Thirty-eight different histidine mutations of Kluyveromyces lactis were iso lated and genetically characterized. All of the mutations were nuclear rece ssive alleles. They turned out to belong to seven different complementation groups, designated hisA1 to hisA7. Five of these genes have been cloned by in vivo complementation of the Klhis mutations. Their homology to some of the histidine genes of Saccharomyces cerevisiae was confirmed by heterologo us complementation. However, one of these KlHIS genes did not complement an y mutation in the seven known histidine biosynthetic enzymes encoding genes (his1-his7) of S. cerevisiae. Copyright (C) 1999 John Wiley & Sons, Ltd.