L. Cicinsain et al., PHYSIOLOGICAL-CHARACTERISTICS OF SEROTONIN TRANSPORTERS ON RAT PLATELETS, Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 120(4), 1998, pp. 723-729
Physiological characteristics of serotonin (5-hydroxytryptamine, 5HT)
transport through the platelet membrane was investigated in Wistar rat
s with our recently developed method permitting repetitive measurement
s of transporter kinetics in individual animals. Full kinetic analysis
in the population of 91 animals revealed Michaelis constant (K-m) of
0.158 +/- 0.025 mu M and maximal velocity (V-max) of 5HT uptake of 225
+/- 32 pmol per 10(8)platelets min(-1) (mean +/- S.D.). Both kinetic
parameters demonstrated normal distribution curves, which for V-max we
re slightly skevied toward higher than average values. No gender effec
t was shown in frequency distributions, mean values and variability of
kinetic parameters. A significant intraindividual correlation between
kinetic parameters was found suggesting compensation at the level of
the plasma membrane. Kinetic parameters were not influenced by age (un
til the middle age) or annual cycle (under laboratory conditions) and
were shown to be fairly stable in time, supporting the view that plate
let 5HT transport kinetics could be a useful biological trait marker.
(C) 1998 Elsevier Science Inc. All rights reserved.