ISOTOPE-DILUTION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - ELECTROSPRAY TANDEM MASS-SPECTROMETRY ASSAY FOR THE MEASUREMENT OF 8-OXO-7,8-DIHYDRO-2'-DEOXYGUANOSINE IN BIOLOGICAL SAMPLES

Authors
RAVANAT JL DURETZ B GUILLER A DOUKI T CADET J
Citation
Jl. Ravanat et al., ISOTOPE-DILUTION HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY - ELECTROSPRAY TANDEM MASS-SPECTROMETRY ASSAY FOR THE MEASUREMENT OF 8-OXO-7,8-DIHYDRO-2'-DEOXYGUANOSINE IN BIOLOGICAL SAMPLES, Journal of chromatography B. Biomedical sciences and applications, 715(2), 1998, pp. 349-356
Citations number
27
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Journal of chromatography B. Biomedical sciences and applications
ISSN journal
13872273 → ACNP
Volume
715
Issue
2
Year of publication
1998
Pages
349 - 356
Database
ISI
SICI code
0378-4347(1998)715:2<349:IHL-E>2.0.ZU;2-#
Abstract
A sensitive and specific assay aimed at measuring 8-oxo-7,8-dihydro-2' -deoxyguanosine (8-oxodGuo) has been developed by associating a revers ed-phase liquid chromatographic separation with an electrospray tandem mass spectrometric detection. The HPLC-MS approach in the single ion monitoring (SIM) mode and the HPLC-MS/MS assay in the multiple reactio n monitoring (MRM) mode have been compared, using isotopically labeled [M+4] 8-oxodGuo as the internal standard. The limit of detection of 8 -oxodGuo was found to be around 5 pmol and 20 fmol for the HPLC-MS and HPLC-MS/MS methods, respectively. The HPLC-MS/MS assay is sensitive e nough to allow the determination of the level of 8-oxodGuo in cellular liver DNA and in urine samples. (C) 1998 Elsevier Science B.V. All ri ghts reserved.