HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY OF (+ -)-ETHOPROPAZINE AND ITS ENANTIOMERS IN RAT PLASMA/

Two high-performance Liquid chromatographic (HPLC) methods are describ ed for determination of (+/-)-ethopropazine (ET) in rat plasma. After deproteination and Liquid-Liquid extraction, assay of (+/-)-ET was per formed using either a C-18 column (non-stereospecific assay) or an (al pha-R-naphthyl)ethylurea column (stereospecific assay). The UV detecti on was at 250 nm. Mean recovery was >85%. Both assays demonstrated exc ellent linear relationships between peak height ratios and plasma conc entrations; quantitation limits were less than or equal to 25 ng/ml, b ased on 100 mu l rat plasma. Accuracy and precision were <17% with bot h methods. Both methods were applied successfully to the measurement o f ET plasma concentrations in rats given the drug intravenously. (C) 1 998 Elsevier Science B.V. All rights reserved.