SIMPLE HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC METHOD FOR DETERMINATION OF KETOCONAZOLE IN HUMAN PLASMA

A simple high-performance liquid chromatographic method using fluoresc ence detection was developed for the determination of ketoconazole in human plasma. The method entailed direct injection of the plasma sampl e after deproteinization using acetonitrile. The mobile phase comprise d 0.05 M disodium hydrogen orthophosphate and acetonitrile (50:50, v/v ) adjusted to pH 6. Analysis was run at a flow-rate of 1.5 ml/min with the detector operating at an excitation wavelength of 260 nm and an e mission wavelength of 375 nm. The method is specific and sensitive wit h a quantification limit of approximately 60 ng/ml and a detection Lim it of 40 ng/ml at a signal-to-noise ratio of 3:1. Mean absolute recove ry value was about 105%, while the within-day and between-day coeffici ent of variation and percent error values of the assay method were all less than 14%. The calibration curve was linear over a concentration range of 62.5-8000 ng/ml. (C) 1998 Elsevier Science B.V. All rights re served.