DEVELOPMENT OF A RAPID METHOD FOR QUANTITATIVE-EVALUATION OF MYCOBACTERIUM-TUBERCULOSIS GROWTH BASED ON COMPETITIVE POLYMERASE-CHAIN-REACTION

Authors
LIGOZZI M PELOSI E FONTANA R
Citation
M. Ligozzi et al., DEVELOPMENT OF A RAPID METHOD FOR QUANTITATIVE-EVALUATION OF MYCOBACTERIUM-TUBERCULOSIS GROWTH BASED ON COMPETITIVE POLYMERASE-CHAIN-REACTION, Journal of Medical Microbiology, 47(10), 1998, pp. 933-936
Citations number
8
Categorie Soggetti
Microbiology
Journal title
Journal of Medical Microbiology → ACNP
ISSN journal
00222615
Volume
47
Issue
10
Year of publication
1998
Pages
933 - 936
Database
ISI
SICI code
0022-2615(1998)47:10<933:DOARMF>2.0.ZU;2-F
Abstract
A competitive polymerase chain reaction (cPCR) assay for the quantitat ive evaluation of Mycobacterium tuberculosis growth was developed base d on co-amplification of genomic DNA and a modified DNA fragment deriv ed from a well-conserved region of the 16S rRNA gene. There was a good correlation between the number of DNA copies in the sample, indicated by competitive PCR, and the number of colony forming units determined by conventional culture methods.