AUTOREGULATION OF THE PTF-FC2 PROTEIC POISON-ANTIDOTE PLASMID ADDICTION SYSTEM (PAS) IS ESSENTIAL FOR PLASMID STABILIZATION

Authors
SMITH ASG RAWLINGS DE
Citation
Asg. Smith et De. Rawlings, AUTOREGULATION OF THE PTF-FC2 PROTEIC POISON-ANTIDOTE PLASMID ADDICTION SYSTEM (PAS) IS ESSENTIAL FOR PLASMID STABILIZATION, Journal of bacteriology (Print), 180(20), 1998, pp. 5463-5465
Citations number
15
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology (Print) → ACNP
ISSN journal
00219193
Volume
180
Issue
20
Year of publication
1998
Pages
5463 - 5465
Database
ISI
SICI code
0021-9193(1998)180:20<5463:AOTPPP>2.0.ZU;2-Z
Abstract
The pasABC genes of the proteic plasmid addiction system of broad-host -range plasmid pTF-FC2 were autoregulated. The PasA antidote was able to repress the operon 25-fold on its own, and repression was increased to 100-fold when the PasB toxin was also present. Autoregulation appe ars to be an essential requirement for pas-mediated plasmid stabilizat ion because when the pas genes were placed behind the isopropyl-beta-D -thiogalactopyranoside (IPTG)-regulated tac promoter, they were unable to stabilize a heterologous test plasmid.