EXPRESSION OF PSEUDOMONAS-AMYLODERAMOSA ISOAMYLASE GENE IN SACCHAROMYCES-CEREVISIAE

Authors
CHEN PH LIN LL HSU WH
Citation
Ph. Chen et al., EXPRESSION OF PSEUDOMONAS-AMYLODERAMOSA ISOAMYLASE GENE IN SACCHAROMYCES-CEREVISIAE, Biotechnology letters, 20(8), 1998, pp. 735-739
Citations number
22
Categorie Soggetti
Biothechnology & Applied Migrobiology
Journal title
Biotechnology letters → ACNP
ISSN journal
01415492
Volume
20
Issue
8
Year of publication
1998
Pages
735 - 739
Database
ISI
SICI code
0141-5492(1998)20:8<735:EOPIGI>2.0.ZU;2-9
Abstract
Isoamylase gene (iso) of Pseudomonas amyloderamosa was amplified by po lymerase chain reaction and cloned into Saccharomyces cerevisiae vecto rs under the control of alcohol dehydrogenase gene and glyceraldehyde- 3-phosphate dehydrogenase gene promoters. The signal sequence of iso g ene was also replaced with that of Schwanniomyces occidentalis alpha-a mylase gene. The extracellular isoamylase activity of transformed Sacc . cerevisiae could reach 86 U ml(-1) after a 4-days cultivation.