IMPROVED PRODUCTION OF A BIOADHESIVE PRECURSOR PROTEIN BY FED-BATCH CULTIVATION OF A RECOMBINANT ESCHERICHIA-COLI WITH A PLYSS VECTOR

In batch cultivation, growth of a recombinant Escherichia coli with an inducible T7 expression system and maximum expression of a bioadhesiv e precursor (BP) protein was similar in the strains with and without t he plasmid vector, pLysS. In fed-batch cultivation, however, the strai n harboring pLysS grew slower and had a lower level of BP protein expr ession than that obtained with the strain without pLysS. This suggests that the presence of pLysS in the T7 expression system strongly affec ts the cell growth and expression of BP protein in high cell density c ultivation.