EXO-GLUCANOLYTIC AND ENDO-GLUCANOLYTIC ACTIVITY OF CELLULASES PURIFIED FROM TRICHODERMA-REESEI

Authors
GAMA FM VILANOVA M MOTA M
Citation
Fm. Gama et al., EXO-GLUCANOLYTIC AND ENDO-GLUCANOLYTIC ACTIVITY OF CELLULASES PURIFIED FROM TRICHODERMA-REESEI, Biotechnology techniques, 12(9), 1998, pp. 677-681
Citations number
12
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
9
Year of publication
1998
Pages
677 - 681
Database
ISI
SICI code
0951-208X(1998)12:9<677:EAEAOC>2.0.ZU;2-6
Abstract
Four cellulases, produced by Trichoderma reesei, have been purified by preparative isoelectric focusing (Rotofor), size exclusion (Sephacryl 100 HR), anionic (Mono Q) and cationic (Mono S) chromatography and ch romatofocusing (Mono P). Enzymatic activity with a large number of sub strates allowed the proteins to be classified as: cellobiohydrolase I, cellobiohydrolase II, endoglucanase I and endoglucanase II. The exo- or endo-glucanase character of these enzymes was analysed by using a t echnique based on the measurement of the Avicel insoluble fibres reduc ing power.