STABILIZING CRUDE AND ULTRAFILTRATED ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE FROM ASPERGILLUS-ORYZAE BY TREHALOSE

Authors
TEREBIZNIK MR ZYLBERMAN V BUERA MP PILOSOF AMR
Citation
Mr. Terebiznik et al., STABILIZING CRUDE AND ULTRAFILTRATED ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE FROM ASPERGILLUS-ORYZAE BY TREHALOSE, Biotechnology techniques, 12(9), 1998, pp. 683-687
Citations number
13
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
9
Year of publication
1998
Pages
683 - 687
Database
ISI
SICI code
0951-208X(1998)12:9<683:SCAUAA>2.0.ZU;2-P
Abstract
Thermal resistance of freeze-dried alpha-amylase and alpha-glucosidase in trehalose matrices (1 to 20 % w/v) stored at 90 degrees C and rela tive humidities (RH) between 0 and 44 % was studied. At RH values up t o 33 %, 10 % (w/v) trehalose was necessary to retain at least 50 % of alpha-amylase activity. For alpha-glucosidase, 10 % (w/v) trehalose wa s effective only at 0 % RH. Ultrafiltration of the crude enzymatic fer mentation extracts enhanced enzyme stability per se. However, ultrafil tration in combination with 1 % (w/v) trehalose retained 74 % of alpha -glucosidase and 95 % of alpha-amylase activities.