CHEMICAL PERMEABILIZATION AND IN-SITU REMOVAL OF DAIDZEIN FROM BIOLOGICALLY VIABLE SOYBEAN (GLYCINE-MAX) SEEDS

After 24 h of chemical permeabilization with 20% (v/v) methanol at 25 degrees C, the amount of daidzein released from soybean seeds is 15 to 20% of the amount (0.0423 +/- 0.0045 mg/g seed dry wt) obtained by ph ysical grinding. With this chemical permeabilization condition, 70% of the permeabilized seeds are still able to germinate. The release of d aidzein is enhanced to 33% with the addition of XAD-4 to 20% (v/v) met hanol without affecting seed viability.