STRUCTURES OF NOVEL ACIDIC GALACTOOLIGOSACCHARIDES SYNTHESIZED BY BACILLUS-CIRCULANS BETA-GALACTOSIDASE

The structures of acidic oligosaccharides synthesized by a transglycos ylation reaction by Bacillus circulans beta-galactosidase, using lacto se as the galactosyl donor, and N-acetylneuraminic acid (NeuAc) and gl ucuronic acid (GlcUA) as the accepters were investigated. Acidic oligo saccharides thus synthesized were purified by anion exchange chromatog raphy and charcoal chromatography, The MS and NMR studies indicated th at the acidic oligosaccharides from NeuAc were Gal beta-(1-->8)-NeuAc, Gal beta-(1-->9)-NeuAc, and Gal beta-(1-->3)-Gal beta-(1-->8)-NeuAc, and those from GlcUA were Gal beta-(1-->3)-GlcUA and Gal beta-(1-->4)- Gal beta(1-->3)-GlcUA. These are novel acidic galactooligosaccharides.