STABILITY OF THERMOSTABLE ENZYME, AQUALYSIN-I - A SUBTILISIN-TYPE SERINE-PROTEASE FROM THERMUS-AQUATICUS YT-1

Authors
TANAKA T MATSUZAWA H OHTA T
Citation
T. Tanaka et al., STABILITY OF THERMOSTABLE ENZYME, AQUALYSIN-I - A SUBTILISIN-TYPE SERINE-PROTEASE FROM THERMUS-AQUATICUS YT-1, Bioscience, biotechnology, and biochemistry, 62(9), 1998, pp. 1806-1808
Citations number
14
Categorie Soggetti
Biology,Agriculture,"Biothechnology & Applied Migrobiology","Food Science & Tenology
Journal title
Bioscience, biotechnology, and biochemistry → ACNP
ISSN journal
09168451
Volume
62
Issue
9
Year of publication
1998
Pages
1806 - 1808
Database
ISI
SICI code
0916-8451(1998)62:9<1806:SOTEA->2.0.ZU;2-W
Abstract
We characterized the heat stability and detergent stabilities of aqual ysin I, produced by Thermus aquaticus YT-1, and compared them with tho se of fungal proteinase K and Bacillus subtilisin. Aqualysin I display ed excellent heat and detergent stabilities. Proteinase K, another Cys -containing enzyme, was less stable than aqualysin I. All these enzyme s maintained activities in the presence of urea or Tween-20.