REUSABILITY OF BOND ELUT CERTIFY COLUMNS FOR THE EXTRACTION OF DRUGS FROM PLASMA

The reusability of Bond Elut Certify columns for the extraction of tox icologically relevant drugs from plasma has been evaluated. Pentobarbi tal, hexobarbital, mepivacaine, trimipramine and clonazepam were selec ted as test drugs to represent various classes of drugs. The columns w ere regenerated immediately after an extraction by washing with methan ol, hydrochloric acid (1%), water and methanol, sequentially. These re generated columns were found to be reusable for plasma. More than 85% of the test drugs were recovered when the columns were used three time s. However, the extraction power of the regenerated columns decreased slightly with the number of reuses, so that a column should not be use d more than two or three times.