HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF TRIPTONIDE, TRIPTRIOLIDE, AND TRIPTOPHENOLIDE IN ETHYL-ACETATE EXTRACT OF TRIPTERYGIUM-WILFORDII HOOK F
Yp. Mao et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF TRIPTONIDE, TRIPTRIOLIDE, AND TRIPTOPHENOLIDE IN ETHYL-ACETATE EXTRACT OF TRIPTERYGIUM-WILFORDII HOOK F, Journal of liquid chromatography & related technologies, 21(17), 1998, pp. 2699-2714
Citations number
18
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
To monitor the composition of extracts of the Chinese herbal remedy Tr
ipterygium wilfordii Hook F (TwHF), a rapid selective, and sensitive r
everse phase HPLC method was developed for the quantitative determinat
ion of some of the major or active diterpenoid components, triptonide
(1), triptophenolide (2), and triptriolide (3). Ethyl acetate extracts
; of TwHF were extracted with chloroform, filtered, and then purified
by passage through mixed columns of silica gel and alumina B for 1 and
2, and an alumina B cartridge for 3. The columns were eluted with eth
yl acetate:chloroform, 1:9, for 1 and, 3:7, for 2. The alumina B cartr
idge was washed with ethyl acetate : chloroform, 1 : 9 and then 3 was
eluted with ethyl acetate : methanol, 8:2. The elutions were chromatog
raphed on a silica-based reverse phase HPLC Cls column with the mobile
phase of water : acetonitrile, 67 : 33, 54 : 46, and 86: 14 for 1, 2,
and 3, respectively, with UV detection performed at 218 nm. Linear de
tection responses were observed for the calibration curves in the conc
entration ranges of 0.64-20.48 mg/L for 1, 4.04-60.60 mg/L for 2, and
0.31-19.84 mg/L for 3. The average recoveries were 96.88%, 102.54%, an
d 100.09%, and the limits of quantitation were 0.174 mg/L, 0.049 mg/L,
and 0.112 mg/L for 1, 2, and 3, respectively.