TRANSCRIPTIONAL REGULATION OF HUMAN TRANSCRIPTION FACTOR IIB IN SMMC-7721 HUMAN HEPATOCELLULAR-CARCINOMA CELLS BY ALL-TRANS-RETINOIC ACID AND PHORBOL 12-MYRISTATE 13-ACETATE

The differential-display reverse transcription/polymerase chain reacti on (DDRT-PCR) method was utilised to find genes differentially express ed at the messenger RNA level in SMMC-7721 human hepatocellular carcin oma cells treated and not treated with all-trans-retinoic acid, a diff erentiation inducer of the cell. Human transcription factor IIB (TFIIB ) was discovered to be decreased on the 3rd day after the cells had be en treated with retinoic acid and increased by phorbol 12-myristate 13 -acetate, which stimulated the proliferation of human hepatocellular c arcinoma cells. It was also found that the transcription of TFIIB in S MMC-7721 cell had no relationship to the cell cycle.