ENGINEERING N-GLYCOSYLATION PATHWAYS IN THE BACULOVIRUS-INSECT CELL SYSTEM

The inability to produce eukaryotic glycoproteins with complex N-linke d glycans is a major limitation of the baculovirus-insect cell express ion system. Recent studies have demonstrated that metabolic engineerin g can be used to extend the glycoprotein processing capabilities of le pidopteran insect cells. This approach is being used to develop new ba culovirus-insect cell expression systems that can produce more authent ic recombinant glycoproteins and obtain new information on insect N-gl ycosylation pathways.