HETEROGENEOUS EXPRESSION OF TUMOR-NECROSIS-FACTOR-ALPHA RECEPTOR-I AND RECEPTOR-II ON HUMAN PERIPHERAL EOSINOPHILS

Citation
G. Matsuyama et al., HETEROGENEOUS EXPRESSION OF TUMOR-NECROSIS-FACTOR-ALPHA RECEPTOR-I AND RECEPTOR-II ON HUMAN PERIPHERAL EOSINOPHILS, International archives of allergy and immunology, 117, 1998, pp. 28-33
Citations number
21
Categorie Soggetti
Allergy,Immunology
ISSN journal
10182438
Volume
117
Year of publication
1998
Supplement
1
Pages
28 - 33
Database
ISI
SICI code
1018-2438(1998)117:<28:HEOTRA>2.0.ZU;2-7
Abstract
Background: Expression of the tumor necrosis factor-alpha receptor by human eosinophils has not been determined. We examined the surface exp ression and mRNA for tumor necrosis factor-alpha receptors I and II (T NF-alpha RI and TNF-alpha RII) on peripheral eosinophils. Methods: Eos inophils were obtained from 17 asthma patients and 3 healthy volunteer s. Flow cytometry was used to examine receptor expression, and the rev erse transcriptase-polymerase chain reaction was used to examine recep tor mRNA expression. Results: Flow cytometry revealed that 16 out of 2 0 subjects had eosinophils expressing TNF-alpha RI and 18 subjects had eosinophils expressing TNF-alpha RII. All eosinophils expressed at le ast one receptor. The mRNA for TNF-alpha RII was detected in all eosin ophils examined, but the signals for TNF-alpha RI mRNA were only found in cells expressing this receptor. Conclusions: Human peripheral eosi nophils show heterogeneous expression of TNF-alpha RI and TNF-alpha RI I.