RAPID PURIFICATION OF A LIPASE FROM ACINETOBACTER-CALCOACETICUS BY TEMPERATURE-INDUCED AQUEOUS 2-PHASE SYSTEMS

A full-factorial design was used to assess the influence of ion streng th and Triton X-114 concentration on the extractive purification of a lipase from Acinetobacter calcoaceticus AAC 323-I in temperature-induc ed aqueous two-phase systems. The condition for optimal extraction was found at 0.75 M NaCl and 3% Triton X-114 concentrations, as shown by surface response analysis. Based on these results, a rapid lipase puri fication procedure from culture supernatant was developed. Such proced ure allows direct extraction from culture broths in three successive s teps with no additional operations. This technique enabled us to extra ct 68% of the enzyme and achieved a purification factor of 41.