LIPID ISOLATION AND FATTY-ACID ANALYSIS IN SACCHAROMYCES-CEREVISIAE -COMPARISON OF DIFFERENT METHODS

Authors
LAMACKA M SAJBIDOR J BOHOV P
Citation
M. Lamacka et al., LIPID ISOLATION AND FATTY-ACID ANALYSIS IN SACCHAROMYCES-CEREVISIAE -COMPARISON OF DIFFERENT METHODS, Biotechnology techniques, 12(8), 1998, pp. 621-625
Citations number
10
Categorie Soggetti
Biothechnology & Applied Migrobiology","Biochemical Research Methods
Journal title
Biotechnology techniques → ACNP
ISSN journal
0951208X
Volume
12
Issue
8
Year of publication
1998
Pages
621 - 625
Database
ISI
SICI code
0951-208X(1998)12:8<621:LIAFAI>2.0.ZU;2-J
Abstract
Several methods for lipid isolation and fatty acid analysis in two dif ferent strains of Saccharomyces cerevisiae were compared. As the most effective procedure the extraction with tetrachlormethane-diethyl ethe r-petrol ether mixture with an acid digestion step followed by fatty a cid analysis by capillary GC was found. Other methods underestimated b oth the lipid content and the content of fatty acids.