SIMPLE AND EFFICIENT PROTOCOL FOR ISOLATION OF HIGH-MOLECULAR-WEIGHT DNA FROM STREPTOMYCES-AUREOFACIENS

A simple and efficient protocol involving the use of cetyltrimethylamm onium bromide (CTAB) for rapid isolation of high molecular wt (greater than or equal to 50 kb) DNA from Streptomyces aureofaciens is describ ed. The DNA yields range from 1.5-2.5 mg per 1.0 g (wet wt) of mycelia , with the purity measured at A(260/280) of 1.83-1.97 and also at A(26 0/230) of 2.2-2.71. The DNA preparation is suitable as substrate for r estriction digestion, Southern hybridization and library construction.