The newly designed nutrient mist bioreactor provided an environment su
pporting multiple-layer culture. In vitro tubers of potato (Solanum tu
berosum L.) were induced and cultured in this bioreactor by using two
culture methods. The percentage of the inocula eventually forming tube
rs in a two-step method was 98% with only 54% being formed in a one-st
ep method. The two-step method improved the efficiency of tuber induct
ion, expedited and synchronized the tuberization.