EFFECT OF CARBON SOURCE ON PRODUCTION OF ALPHA-L-ARABINOFURANOSIDASE BY AUREOBASIDIUM-PULLULANS

A color-variant strain of Aureobasidium pullulans (NRRL Y-12974) produ ced alpha-L-arabinofuranosidase (alpha-L-AFase) when grown in liquid c ulture on sugar beet arabinan, wheat arabino-xylan, L-arabinose, L-ara bitol, xylose, xylitol, oat spelt xylan, corn fiber, or arabinogalacta n. L-Arabinose was most effective for production of both whole-broth a nd extracellular alpha-L-AFase activity, followed by L-arabitol. Oat s pelt xylan, sugar beet arabinan, xylose, xylitol, and wheat arabinoxyl an were intermediate in their ability to support alpha-L-AFase product ion. Lower amounts of enzyme activity were detected in corn fiber- and arabinogalactan-grown cultures.