Following 15 yr of successful use, glyphosate failed to control a popu
lation of the widespread grass weed rigid ryegrass in Australia. This
population proved to be resistant to glyphosate in pot dose-response e
xperiments conducted outdoors, exhibiting 7- to 11-fold resistance whe
n compared to a susceptible population. Some cross-resistance to diclo
fop-methyl (about 2.5-fold) was also observed. Similar levels. of cont
rol of the resistant and susceptible populations were obtained followi
ng application of amitrole, chlorsulfuron, fluazifop-P-butyl, paraquat
, sethoxydim, simazine, or tralkoxydim. The presence of glyphosate res
istance in a major weed species indicates a need for changes in glypho
sate use patterns.