A bioassay using red beet root length indicated an increase in imazeth
apyr bioactivity in Berryland sand (BLS) as soil pH increased from 3.7
to 6.5. Increasing pH above 6.5 had no effect on imazethapyr bioactiv
ity. The lowest imazethapyr concentrations detected by the bioassay at
pH 6.5 were 0.5, 1, 2.5, and 5 to 10 mu g kg(-1) in acid-washed quart
z sand, BLS, Aura loamy sand, and muck soil, respectively After applic
ation of 0.07 kg ae ha(-1) with 12.5 mm of simulated rainfall at 3-d i
ntervals, imazethapyr remained in the 0 to 15 cm soil in BLS soil colu
mns. Under this rainfall regime, the herbicide moved 30 cm deep 5 mo a
fter application, but residues decreased significantly in surface soil
. Imazethapyr bioactivity was highest and mobility was lowest at low s
oil temperature (10 C) and low moisture (4 to 6% w/w). Imazethapyr mov
ement was monitored successfully using the red beet root length bioass
ay, and an herbicide residue map was developed to show the pattern of
imazethapyr bioactivity in soil columns.