REPLACEMENT OF DIETARY SATURATED BY UNSATURATED FATTY-ACIDS - EFFECTSON PLATELET PROTEIN-KINASE-C ACTIVITY, URINARY CONTENT OF 2,3-DINOR-TXB2 AND IN-VITRO PLATELET-AGGREGATION IN HEALTHY MAN
Am. Turpeinen et al., REPLACEMENT OF DIETARY SATURATED BY UNSATURATED FATTY-ACIDS - EFFECTSON PLATELET PROTEIN-KINASE-C ACTIVITY, URINARY CONTENT OF 2,3-DINOR-TXB2 AND IN-VITRO PLATELET-AGGREGATION IN HEALTHY MAN, Thrombosis and haemostasis, 80(4), 1998, pp. 649-655
The present study was conducted to compare the effects of oleic (OA) a
nd linoleic acids (LA) on platelet function in healthy subjects. After
a 4-week period on a diet rich in saturated fatty acids (SAFA), 38 vo
lunteers (20 female, 18 male) had a high OA (18.0 en%) diet or a high
LA diet (11.5 en%) for four weeks in a controlled manner. A control gr
oup of 13 subjects consumed their habitual diet throughout the study.
Replacing the SAFA diet by the OA or LA diet did not affect the membra
ne-associated activity of platelet protein kinase C (PKC). However, bo
th diets tended to increase the cytosolic activity to a comparable ext
ent. Both the OA and the LA diets increased urinary excretion of 2,3-d
inor-TXB2, as compared to the SAFA diet, whereas the urinary excretion
of beta-thromboglobulin remained unchanged. As compared to the initia
l SAFA diet, platelet aggregation to collagen increased after both die
ts, while ADP induced aggregation showed no diet-induced changes. The
results indicate increased platelet activity after both oil diets with
no differences between the OA and LA diet and confirm in vitro findin
gs that cis-unsaturated fatty acids have a distinct effect on cytosoli
c PKC, in particular.