ADSORPTION CHARACTERISTICS OF CELLULASES FROM A CONSTITUTIVE MUTANT OF TRICHODERMA-REESEI

The adsorption parameter, A(max) (maximum protein adsorbed/g substrate ) and K-A (adsorption equilibrium constant) of Trichoderma reesei C-5 cellulases were 58.8 mg protein/g cellulose and 11.6 x 10(4) l/mol res pectively. The activation energies for the adsorption rate constants o f cellobiohydrolases and endoglucanases of C-5 were 30% and 11% lower respectively than that in the parent T. reesei QM9414 enzymes indicati ng the greater binding ability of the former. This was also reflected in its increased saccharification efficiency.