SPHINGOMONAS SP. A1 LYASE ACTIVE ON BOTH POLY-BETA-D-MANNURONATE AND HETEROPOLYMERIC REGIONS IN ALGINATE

Authors
HASHIMOTO W OKAMOTO M HISANO T MOMMA K MURATA K
Citation
W. Hashimoto et al., SPHINGOMONAS SP. A1 LYASE ACTIVE ON BOTH POLY-BETA-D-MANNURONATE AND HETEROPOLYMERIC REGIONS IN ALGINATE, Journal of fermentation and bioengineering, 86(2), 1998, pp. 236-238
Citations number
14
Categorie Soggetti
Food Science & Tenology","Biothechnology & Applied Migrobiology
Journal title
Journal of fermentation and bioengineering → ACNP
ISSN journal
0922338X
Volume
86
Issue
2
Year of publication
1998
Pages
236 - 238
Database
ISI
SICI code
0922-338X(1998)86:2<236:SSALAO>2.0.ZU;2-S
Abstract
Alginate lyase III of Sphingomonas sp. A1 cleaved the glycosidic linka ge of polymannuronate and heteropolymeric region composed of mannurona te and guluronate, but was inert on polyguluronate, The enzyme was obs erved to act endolytically, interact with tetrasaccharide in alginate, and form di- and trisaccharides as final products. This result sugges ts that the enzyme recognizes the unit of tetrasaccharide in alginate and cleaves the middle linkage of the tetrasaccharide.