G. Capranico et M. Binaschi, DNA-SEQUENCE SELECTIVITY OF TOPOISOMERASES AND TOPOISOMERASE POISONS, Biochimica et biophysica acta, N. Gene structure and expression, 1400(1-3), 1998, pp. 185-194
Chemical agents able to interfere with DNA topoisomerases are widespre
ad in nature, and some of them have clinical efficacy as antitumor or
antibacterial drugs. Drugs which have as a target DNA topoisomerases c
ould be divided into two categories: poisons and catalytic inhibitors.
Classical topoisomerase poisons stimulate cleavage in a sequence-sele
ctive manner, yielding drug-specific cleavage intensity pattern. The m
echanisms of drug interaction with DNA topoisomerases, the DNA sequenc
e selectivity of the action of topoisomerase II poisons and the identi
fication of structural determinants of their activity have suggested t
hat topoisomerase II poisons may fit into a specific pharmacophore, co
nstituted by a planar ring system with DNA intercalation or intercalat
ion-like properties, and protruding side chains interfering with the p
rotein side of the covalent enzyme-DNA complex. The complete definitio
n of the diverse pharmacophores of topoisomerase II poisons will certa
inly be of value for the design of new agents directed to specific gen
omic sites, and more effective in the treatment of human cancer. 0167-
4781/98/$ - see front matter (C) 1998 Elsevier Science B.V. All rights
reserved.