The composition of the dominating populations within a bacterioplankto
n community was investigated in a mesotrophic, boreal forest lake. Com
posite samples were collected monthly throughout the lake for two year
s. The community composition was determined by denaturing gradient gel
electrophoresis (DGGE) of a polymerase chain reaction (PCR)-amplified
part of 16S rDNA, extracted from organisms smaller than 1 mu m. Tempo
ral patterns of occurrence in the lake differed among populations. The
re was no clear seasonal pattern of variation, but there was a gradual
change. The results suggest that variation in the amount of water flo
wing into the lake could explain some of the changes in the bacteriopl
ankton community. (C) 1998 Federation of European Microbiological Soci
eties. Published by Elsevier Science B.V. All rights reserved.