The general acceptance of bioremediation technology as an environmenta
lly sound and economic treatment for hazardous waste requires the demo
nstration of its efficacy, reliability and predictability, as well as
its advantages over conventional treatments. An effective monitoring d
esign includes protocols for treatment-specific, representative sampli
ng, control and monitoring: these should take into account abiotic and
biotic pollutant fate processes in all relevant process compartments.
A number of well-established and novel chemical and molecular biologi
cal monitoring techniques and parameters are available. Logical and ba
lanced combinations of both chemical and biological monitoring paramet
ers should be used to demonstrate complete degradation and detoxificat
ion of a hazardous waste as well as the biological nature of the proce
ss. At each process-scale level, a set of general criteria should be u
sed for a systematic evaluation of the overall efficacy of bioremediat
ion.