NONAQUEOUS ENZYMOLOGY APPROACH FOR IMPROVEMENT OF REAGENTLESS MEDIATOR-BASED GLUCOSE BIOSENSOR

Authors
LUKACHOVA LV KARYAKIN AA IVANOVA YN KARYAKINA EE VARFOLOMEYEV SD
Citation
Lv. Lukachova et al., NONAQUEOUS ENZYMOLOGY APPROACH FOR IMPROVEMENT OF REAGENTLESS MEDIATOR-BASED GLUCOSE BIOSENSOR, Analyst (London. 1877. Print), 123(10), 1998, pp. 1981-1985
Citations number
18
Categorie Soggetti
Chemistry Analytical
Journal title
Analyst (London. 1877. Print) → ACNP
ISSN journal
00032654
Volume
123
Issue
10
Year of publication
1998
Pages
1981 - 1985
Database
ISI
SICI code
0003-2654(1998)123:10<1981:NEAFIO>2.0.ZU;2-G
Abstract
The formation of Nafion membranes containing glucose oxidase and dimet hylferrocene as a mediator was optimized using a previously reported n on-aqueous enzymology approach for biosensor development. Enzyme immob ilization in Nafion membranes was carried out from water-organic mixtu res with a high content of organic solvent. The mediator based reagent less glucose electrode was tested in a flow injection system. The resp onse towards glucose addition was stable: the reproducibility during 5 0 assays exceeded 95%. The response was linear over the glucose concen tration range 0.5-50 mM.