TRILINEAR PARAFAC DECOMPOSITION OF SYNCHRONOUS FLUORESCENCE-SPECTRA OF MIXTURES OF THE MAJOR METABOLITES OF ACETYLSALICYLIC-ACID

Mixtures of the three major metabolites of acetylsalicylic acid (salic ylic, gentisic and salicyluric acid) were analyzed by synchronous mole cular fluorescence spectroscopy. Three-way data matrices were generate d by acquisition of spectra as a function bf the pH (between 2 and 11) and of different relative concentrations of the three components. The PARAFAC trilinear model, without restrictions and using one factor pe r metabolite, was used in the data analysis. A full decomposition of t he data matrices into the spectra, concentration and pH profiles was o btained. This result shows that molecular fluorescence spectroscopy ca n be used for the development of robust analytical methods for th simu ltaneous determination of the three major metabolites of acetylsalicyl ic acid in complex background samples.