MOLECULAR-CLONING AND SEQUENCE-ANALYSIS OF THE SCHIZOSACCHAROMYCES-POMBE ADE10(+) GENE

We have cloned and sequenced the Schizosaccharomyces pombe ade10 gene encoding 5-phosphoribosyl-4carboxamide 5-aminoimidazole transformylase inosine monophosphate cyclohydrolase. The sequence has an uninterrupt ed open reading frame of 1755 nucleotides corresponding to 585 amino a cid residues. The deduced amino acid sequence shows a high degree of s imilarity to the purH gene product of many species, including Saccharo myces cerevisiae, human, chicken and Escherichia coli. Moreover our da ta indicate that intrachromosomal recombination in Schiz. pombe is enh anced if the ade10 gene product is defective. The sequence has been su bmitted to the EMBL ;data library under Accession Number Y16419. (C) 1 998 John Wiley & Sons, Ltd.