IMMOBILIZATION OF DNA ON SILVER SURFACE OF BULK ACOUSTIC-WAVE SENSOR AND ITS APPLICATION TO THE STUDY OF UV-C DAMAGE

A simple, inexpensive, on-line bulk acoustic wave (BAW) DNA biosensor is proposed. DNA is immobilized onto an Ag-plated surface rather than the conventional Au surface. Thioglycollic (TGC) acid is used as an ac tive coating for DNA immobilization. The immobilization of DNA and the different binding behavior of ds-DNA and ss-DNA are investigated. The result of the enzyme immunoassay also verifies that DNA is successful ly immobilized onto the Ag surface. The experimental relationship betw een af, and the amount of added DNA on the TGC acid modified BAW senso r surface is presented. This novel DNA biosensor is used to monitor th e whole process of W-C induced DNA damage in vitro and to get the real -time frequency response curves. Results show that the W-C damage in a queous solution exhibits an apparent tendency towards strand breaks. ( C) 1998 Elsevier Science B.V. All rights reserved.