THIDIAZURON-INDUCED SHOOT REGENERATION IN PIGEONPEA (CAJANUS-CAJAN L.)

Thidiazuron either alone or in combination with IAA induced high frequ ency shoot regeneration from primary leaf segments of three pigeonpea cultivars. Transfer of the cultures to medium with reduced concentrati on of thidiazuron resulted in further development of the shoots. The r egenerated shoots were subsequently transferred to medium supplemented with BA, IAA and gibberellic acid where 5-10% of the shoots elongated further. Rooting of shoots could be obtained on half strength MS medi um supplemented with NAA. Histological studies confirmed the mode of r egeneration as shoot organogenesis.