INTRON BASED RADIATION HYBRID MAPPING OF 15 COMPLEX-I GENES OF THE HUMAN ELECTRON-TRANSPORT CHAIN

At least 34 complex I subunits of the electron transport chain are enc oded by the nuclear genome, but only 14 of these have been mapped in t he human. To rapidly map additional subunits, we have performed a comb ination of database mining and direct ''wet'' experimentation to ident ify intron and/or 5' upstream genomic DNA regions for 16 complex I gen es. Wet experimentation was applied to 5 genes, and involved direct PC R amplification of introns by inter-exon PCR or splinkerette based PCR walking. Database mining was applied to 11 genes, and entailed the id entification of incompletely spliced mRNAs and genomic CpG island clon e sequences. This data was in files that carried no documentary descri ption of the non-exon regions. Non-exon sequences were thus derived fo r 15 complex I genes and used to design functional gene specific PCR a ssays. Radiation hybrid mapping of these PCRs located 15 complex I gen es to chromosomes 1, 4, 5 (2 genes), 7 (2 genes), 8, 9 (2 genes), 11, 14, 16 (2 genes), 18, and 19.