H. Gantelet et al., CHARACTERISTICS OF PULLULANASES FROM EXTREMELY THERMOPHILIC ARCHAEA ISOLATED FROM DEEP-SEA HYDROTHERMAL VENTS, Biotechnology letters, 20(9), 1998, pp. 819-823
Two out of three extremely thermophilic anaerobic archaea, isolated fr
om deep-sea hydrothermal vents, produced pullulanase activity in the p
resence of maltose in the growth medium. Enzyme activities were mainly
extracellular and characterized by optimum temperatures of 95 degrees
C and 80-95 degrees C, optimum pH of 5.0-7.0 and a high degree of the
rmostability. One strain when grown in a fermenter with maltose as ind
ucer produced pullulanase at 35 U/I.