The response of Lactobacillus acidophilus CRL 639 to cold-shock (freez
ing) depends on the initial growth temperature. Cells cultured at 25 d
egrees C (long filaments with a mu value of 0.02 h(-1)) show a higher
beta-galactosidase activity and develop cryotolerance, compared with c
ells grown at 37 degrees C. One-dimensional proteins gel electrophores
is shows the overexpression of six proteins (22.5-105.0 kDa) and the r
epression of eight proteins (4.3-48.0 kDa) at 25 degrees C.