R. Chiba et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC DETERMINATION OF YOHIMBINE INSERUM BY CHEMILUMINESCENCE DETECTION, Analytical sciences, 14(5), 1998, pp. 975-978
An ion-pair high-performance liquid chromatography with chemiluminesce
nce detection using tris(2,2'-bipyridyl)ruthenium(II) [Ru(bpy)(3)(2+)]
was developed for the determination of yohimbine (YOH) in human serum
. A sample solution, containing narceine as an internal standard, was
treated with a Bond-Elut Certify disposable column, and the purified f
raction was separated on a TSKgel ODS-80Ts column. The recovery of YOH
at 50 ng/ml was 97.2+/-4.4% (mean+/-SD, n=5). The detection limit was
calculated to be 30 ng/ml of YOH in serum (S/N=3). The method was suc
cessfully applied to drug monitoring of the YOH level in the serum of
healthy volunteers.