T. Yamakuni et al., A NOVEL PROTEIN CONTAINING CDC10 SWI6 MOTIFS REGULATES EXPRESSION OF MESSENGER-RNA ENCODING CATECHOLAMINE BIOSYNTHESIZING ENZYMES/, The Journal of biological chemistry, 273(42), 1998, pp. 27051-27054
Catecholaminergic (dopaminergic, noradrenergic, and adrenergic) transm
itter phenotypes require the cooperative actions of four biosynthetic
enzymes: tyrosine hydroxylase, aromatic L-amino acid decarboxylase, do
pamine beta-hydroxylase, and phenylethanolamine N-methyltransferase, M
echanisms that control expression of these enzymes in a transmitter ph
enotype-specific manner, however, are poorly understood. Here, we prov
ide evidence that overexpression of a novel cdc10/SWI6 motif-containin
g protein, V-1, elicits the coordinate up regulation of tyrosine hydro
xylase, aromatic L-amino acid decarboxylase, and dopamine beta-hydroxy
lase mRNAs in the neuronal cell line PC12D, and as a result, catechola
mine levels are increased. Furthermore, V-1 is strongly expressed in t
he cytoplasm of rat chromaffin cells of adrenal medulla, Thus, V-1 may
act as a cytoplasmic protein/protein adapter and be involved in contr
ol of the catecholaminergic phenotype expression via an intracellular
pathway signaling to the nucleus.